{"id":12863,"date":"2026-10-23T20:42:29","date_gmt":"2026-10-23T20:42:29","guid":{"rendered":"https:\/\/mycosupplies.eu\/2026\/10\/23\/still-air-box\/"},"modified":"2026-11-13T14:10:09","modified_gmt":"2026-11-13T14:10:09","slug":"still-air-box","status":"publish","type":"post","link":"https:\/\/mycosupplies.eu\/cs\/2026\/10\/23\/still-air-box\/","title":{"rendered":"Still air box: jak ji postavit a pou\u017e\u00edvat p\u0159i p\u011bstov\u00e1n\u00ed hub"},"content":{"rendered":"<p><strong>Still air box<\/strong> (SAB, box se stoj\u00edc\u00edm vzduchem) je nejlevn\u011bj\u0161\u00ed zp\u016fsob, jak p\u0159i p\u011bstov\u00e1n\u00ed hub pracovat \u010dist\u011b. Je to jednodu\u0161e pr\u016fhledn\u00fd plastov\u00fd box se dv\u011bma otvory na ruce. Uvnit\u0159 boxu se vzduch neh\u00fdbe, tak\u017ee spory a bakterie klesaj\u00ed ke dnu, m\u00edsto aby dopadaly na va\u0161i pr\u00e1ci. V tomto n\u00e1vodu se dozv\u00edte, jak still air box postavit, jak ji pou\u017e\u00edvat a kdy je lep\u0161\u00ed volbou lamin\u00e1rn\u00ed box.<\/p>\n<h2>Co je still air box?<\/h2>\n<p>Still air box je uzav\u0159en\u00fd pr\u016fhledn\u00fd box se dv\u011bma otvory pro ruce, kter\u00fd v\u00e1m d\u00e1v\u00e1 malou z\u00f3nu klidn\u00e9ho vzduchu pro pr\u00e1ci. Proto\u017ee j\u00edm nic neproud\u00ed, ne\u010distoty ze vzduchu klesaj\u00ed ke dnu, m\u00edsto aby dopadaly na otev\u0159en\u00e9 misky, sklenice nebo s\u00e1\u010dky. V\u011bt\u0161ina dom\u00e1c\u00edch p\u011bstitel\u016f pou\u017e\u00edv\u00e1 \u00falo\u017en\u00fd box o objemu 60 a\u017e 100 litr\u016f, do kter\u00e9ho se vejde stoh Petriho misek a s\u00e1\u010dek se zrnem.<\/p>\n<h2>Pro\u010d je steriln\u00ed pr\u00e1ce d\u016fle\u017eit\u00e1<\/h2>\n<p>V\u011bt\u0161ina kontaminac\u00ed poch\u00e1z\u00ed ze vzduchu kolem v\u00e1s. Ka\u017ed\u00fd pohyb zv\u00ed\u0159\u00ed prach, spory pl\u00edsn\u00ed a bakterie. Box tento pohybuj\u00edc\u00ed se vzduch zadr\u017e\u00ed. Proto m\u016f\u017eete agarovou misku nebo sklenici na chv\u00edli otev\u0159\u00edt s mnohem men\u0161\u00edm rizikem kontaminace. Pro za\u010d\u00e1te\u010dn\u00edky je to nejlep\u0161\u00ed prvn\u00ed krok p\u0159ed n\u00e1kupem drah\u00e9ho vybaven\u00ed.<\/p>\n<h2>Still air box: jak ji postavit<\/h2>\n<p>Pot\u0159ebujete jen p\u00e1r jednoduch\u00fdch v\u011bc\u00ed:<\/p>\n<ul>\n<li>Pr\u016fhledn\u00fd plastov\u00fd \u00falo\u017en\u00fd box o objemu zhruba 60\u2013100 litr\u016f<\/li>\n<li>Fix a plechovku nebo kulatou \u0161ablonu o pr\u016fm\u011bru asi 10 cm<\/li>\n<li>Roz\u017ehaven\u00fd n\u016f\u017e, p\u00e1je\u010dku nebo vrtac\u00ed korunku<\/li>\n<li>70% isopropylalkohol a pap\u00edrov\u00e9 ut\u011brky<\/li>\n<\/ul>\n<p>Nejprve box oto\u010dte dnem vzh\u016fru, aby se z v\u00edka stalo dno. Potom na del\u0161\u00ed stran\u011b nakreslete dva kruhy, zhruba na \u0161\u00ed\u0159ku dlan\u011b od sebe a v polovin\u011b v\u00fd\u0161ky. N\u00e1sledn\u011b otvory pomalu vy\u0159\u00edzn\u011bte hork\u00fdm no\u017eem. Nakonec okraje uhla\u010fte, aby v\u00e1m neprotrhly rukavice. N\u011bkte\u0159\u00ed p\u011bstitel\u00e9 p\u0159id\u00e1vaj\u00ed i ruk\u00e1vy, aby box t\u011bsnil je\u0161t\u011b l\u00e9pe.<\/p>\n<h2>Still air box krok za krokem: jak ji pou\u017e\u00edvat<\/h2>\n<ol>\n<li>Vnit\u0159ek boxu ot\u0159ete 70% alkoholem.<\/li>\n<li>Ot\u0159ete ka\u017edou v\u011bc, kterou dovnit\u0159 vlo\u017e\u00edte, nap\u0159\u00edklad misky, st\u0159\u00edka\u010dky a s\u00e1\u010dky.<\/li>\n<li>Nasa\u010fte si <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/nitrilove-rukavice-nepudrovane\/\">nitrilov\u00e9 rukavice<\/a> a <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/respirator-ffp2-pro-sterilni-praci\/\">respir\u00e1tor<\/a> a rukavice pak post\u0159\u00edkejte alkoholem.<\/li>\n<li>Vlo\u017ete ruce dovnit\u0159 a p\u00e1r minut po\u010dkejte, a\u017e se vzduch uklidn\u00ed.<\/li>\n<li>Pracujte pomalu a misky i sklenice m\u011bjte otev\u0159en\u00e9 co nejkrat\u0161\u00ed dobu.<\/li>\n<\/ol>\n<p>Bezpe\u010dnostn\u00ed tip: v boxu nikdy nepou\u017e\u00edvejte otev\u0159en\u00fd ohe\u0148. V\u00fdpary alkoholu se hromad\u00ed v uzav\u0159en\u00e9m prostoru a mohou vzplanout. M\u00edsto opalov\u00e1n\u00ed jehel pou\u017e\u00edvejte p\u0159edem sterilizovan\u00e9 jehly.<\/p>\n<h2>Co m\u016f\u017eete v SAB d\u011blat?<\/h2>\n<p>SAB se hod\u00ed na mal\u00e9, rychl\u00e9 \u00fakoly. M\u016f\u017eete v n\u00ed nap\u0159\u00edklad nal\u00e9vat nebo o\u010dkovat <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/petriho-miska-sladovo-kvasnicovy-agar\/\">Petriho misky s agarem<\/a>, p\u0159en\u00e1\u0161et mycelium mezi miskami a natahovat st\u0159\u00edka\u010dku z <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/lahvicka-na-tekutou-kulturu-10-ml\/\">lahvi\u010dky s tekutou kulturou<\/a>. Nav\u00edc je to dobr\u00e9 m\u00edsto pro vst\u0159ikov\u00e1n\u00ed <a href=\"https:\/\/mycosupplies.eu\/cs\/kategorie-produktu\/tekuta-kultura\/\">tekut\u00e9 kultury<\/a> do s\u00e1\u010dk\u016f se zrnem p\u0159es injek\u010dn\u00ed port.<\/p>\n<h2>Still air box, nebo lamin\u00e1rn\u00ed box?<\/h2>\n<p>Still air box udr\u017euje vzduch v klidu. Lamin\u00e1rn\u00ed box naopak fouk\u00e1 nad va\u0161i pr\u00e1ci st\u00e1l\u00fd proud vzduchu filtrovan\u00e9ho p\u0159es HEPA. Tento \u010dist\u00fd proud odh\u00e1n\u00ed ne\u010distoty, i kdy\u017e se pohybujete. Proto m\u016f\u017eete pracovat voln\u011b, nechat misky otev\u0159en\u00e9 d\u00e9le a zpracov\u00e1vat v\u011bt\u0161\u00ed d\u00e1vky.<\/p>\n<ul>\n<li><strong>Still air box:<\/strong> velmi levn\u00e1 a snadno postaviteln\u00e1, ale mal\u00e1 a pr\u00e1ce v n\u00ed je pomal\u00e1.<\/li>\n<li><strong>Lamin\u00e1rn\u00ed box:<\/strong> rychl\u00fd, prostorn\u00fd a velmi \u010dist\u00fd, ale dra\u017e\u0161\u00ed.<\/li>\n<\/ul>\n<p>Mnoho p\u011bstitel\u016f za\u010d\u00edn\u00e1 se SAB a p\u0159ejde na lep\u0161\u00ed vybaven\u00ed, jakmile d\u011bl\u00e1 hodn\u011b zrnov\u00e9ho podhoub\u00ed nebo pr\u00e1ce s agarem. Pokud to plat\u00ed i pro v\u00e1s, pod\u00edvejte se na na\u0161i <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/filtracni-jednotka-hepa-envirco-mac-10\/\">filtra\u010dn\u00ed jednotku Envirco MAC 10<\/a> nebo celou nab\u00eddku <a href=\"https:\/\/mycosupplies.eu\/cs\/kategorie-produktu\/laminarni-box\/\">lamin\u00e1rn\u00edch box\u016f<\/a>.<\/p>\n<h2>Funguje still air box opravdu?<\/h2>\n<p>Ano, pro mal\u00e9 a rychl\u00e9 \u00fakoly funguje SAB velmi dob\u0159e. Mnoho p\u011bstitel\u016f v n\u011bm roky prov\u00e1d\u00ed \u010dist\u00e9 p\u0159enosy na agaru a o\u010dkov\u00e1n\u00ed zrna. Pom\u00e1h\u00e1 v\u0161ak jen tehdy, kdy\u017e m\u00e1te dobrou techniku. Nefiltruje vzduch, a nem\u016f\u017ee tedy odstranit ne\u010distoty, kter\u00e9 u\u017e jsou uvnit\u0159. V\u00fdsledek z\u00e1vis\u00ed na t\u0159ech v\u011bcech: d\u016fkladn\u00e9m ot\u0159en\u00ed, pomal\u00fdch pohybech a kr\u00e1tk\u00e9m otev\u00edr\u00e1n\u00ed. U velk\u00fdch d\u00e1vek nebo dlouh\u00e9 pr\u00e1ce se naopak vyplat\u00ed lamin\u00e1rn\u00ed box.<\/p>\n<h2>Koupit, nebo postavit still air box?<\/h2>\n<p>M\u016f\u017eete si koupit hotov\u00e9 boxy, nap\u0159\u00edklad skl\u00e1dac\u00ed stany z PVC s ruk\u00e1vy. Hod\u00ed se, pokud m\u00e1te m\u00e1lo m\u00edsta. P\u0159esto box vyroben\u00fd z \u00falo\u017en\u00e9ho boxu stoj\u00ed jen p\u00e1r eur, sn\u00e1ze se \u010dist\u00ed a funguje stejn\u011b dob\u0159e. A\u0165 zvol\u00edte cokoli, d\u016fle\u017eit\u011bj\u0161\u00ed je vybaven\u00ed kolem: steriln\u00ed <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/injekcni-jehla-18g-bd-microlance\/\">jehly 18G<\/a>, nitrilov\u00e9 rukavice a 70% alkohol.<\/p>\n<h2>\u010cast\u00e9 chyby, kter\u00fdm se vyhnout<\/h2>\n<ul>\n<li><strong>P\u0159\u00edli\u0161 rychl\u00e9 pohyby:<\/strong> prudk\u00e9 pohyby rukou rozv\u00ed\u0159\u00ed vzduch v boxu.<\/li>\n<li><strong>Vynech\u00e1n\u00ed ot\u00edr\u00e1n\u00ed:<\/strong> ka\u017ed\u00fd povrch a ka\u017edou v\u011bc je t\u0159eba o\u010distit alkoholem.<\/li>\n<li><strong>Pr\u00e1ce v pra\u0161n\u00e9 m\u00edstnosti:<\/strong> nepracujte hned po vys\u00e1v\u00e1n\u00ed ani u otev\u0159en\u00e9ho okna.<\/li>\n<li><strong>Otev\u0159en\u00e1 v\u00ed\u010dka:<\/strong> ka\u017edou misku nebo sklenici otev\u00edrejte jen na p\u00e1r sekund.<\/li>\n<\/ul>\n<h2>\u010casto kladen\u00e9 ot\u00e1zky<\/h2>\n<p><strong>Pot\u0159ebujete k p\u011bstov\u00e1n\u00ed hub SAB?<\/strong><\/p>\n<p>Ne pro hotov\u00fd growkit. \u010cist\u00e9 podm\u00ednky ale pot\u0159ebujete, jakmile pracujete s agarem, otev\u00edr\u00e1te sklenice nebo o\u010dkujete zrno. Pak je box s otvory pro ruce nejlevn\u011bj\u0161\u00ed \u0159e\u0161en\u00ed.<\/p>\n<p><strong>Mohu SAB pou\u017e\u00edt pro tekutou kulturu?<\/strong><\/p>\n<p>Ano. Nata\u017een\u00ed st\u0159\u00edka\u010dky z lahvi\u010dky s tekutou kulturou a o\u010dkov\u00e1n\u00ed s\u00e1\u010dk\u016f se zrnem p\u0159es injek\u010dn\u00ed port jsou pro SAB ide\u00e1ln\u00ed \u00fakoly.<\/p>\n<p><strong>Jak dlouho m\u00e1m p\u0159ed za\u010d\u00e1tkem \u010dekat?<\/strong><\/p>\n<p>Po ot\u0159en\u00ed boxu a vlo\u017een\u00ed rukou dovnit\u0159 po\u010dkejte dv\u011b a\u017e p\u011bt minut. Vzduch se tak stihne uklidnit.<\/p>\n<p>Chcete se o steriln\u00ed pr\u00e1ci dozv\u011bd\u011bt v\u00edc? P\u0159e\u010dt\u011bte si n\u00e1\u0161 n\u00e1vod na <a href=\"https:\/\/mycosupplies.eu\/cs\/2018\/02\/26\/petriho-miska-s-agarem-navod\/\">p\u011bstov\u00e1n\u00ed kultur hub na Petriho misk\u00e1ch s agarem<\/a>. V\u011bdu za \u010dist\u00fdm proud\u011bn\u00edm vzduchu vysv\u011btluje <a href=\"https:\/\/en.wikipedia.org\/wiki\/Laminar_flow_cabinet\" target=\"_blank\" rel=\"noopener\">\u010dl\u00e1nek na Wikipedii o lamin\u00e1rn\u00edch boxech<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Jak postavit levnou still air box pro steriln\u00ed pr\u00e1ci s houbami, jak ji pou\u017e\u00edvat krok za krokem a kdy p\u0159ej\u00edt na lamin\u00e1rn\u00ed box.<\/p>\n","protected":false},"author":1,"featured_media":4579,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[816],"tags":[],"class_list":["post-12863","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-pestovani"],"featured_image_src":{"landsacpe":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2023\/11\/1000010741-1118x445.jpg",1118,445,true],"list":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2023\/11\/1000010741-463x348.jpg",463,348,true],"medium":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2023\/11\/1000010741-257x300.jpg",257,300,true],"full":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2023\/11\/1000010741.jpg",1118,1307,false]},"yoast_head":"<!-- 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