{"id":12869,"date":"2018-02-26T07:42:56","date_gmt":"2018-02-26T07:42:56","guid":{"rendered":"https:\/\/mycosupplies.eu\/2018\/02\/26\/petriho-miska-s-agarem-navod\/"},"modified":"2026-11-12T09:50:27","modified_gmt":"2026-11-12T09:50:27","slug":"petriho-miska-s-agarem-navod","status":"publish","type":"post","link":"https:\/\/mycosupplies.eu\/cs\/2018\/02\/26\/petriho-miska-s-agarem-navod\/","title":{"rendered":"Petriho miska s agarem: jak p\u011bstovat kultury hub"},"content":{"rendered":"<p>P\u0159em\u00fd\u0161leli jste n\u011bkdy, jak se p\u011bstuj\u00ed a uchov\u00e1vaj\u00ed \u017eiv\u00e9 kultury hub? Odpov\u011bd\u00ed je <strong>Petriho miska s agarem<\/strong>. Pr\u00e1ce s agarem umo\u017e\u0148uje mykolog\u016fm i hobby p\u011bstitel\u016fm uchov\u00e1vat, \u010distit a mno\u017eit sv\u00e9 obl\u00edben\u00e9 kmeny. V tomto n\u00e1vodu v\u00e1s provedeme od spor nebo tk\u00e1n\u011b a\u017e ke zdrav\u00e9, \u010dist\u00e9 kultu\u0159e. Uk\u00e1\u017eeme v\u00e1m tak\u00e9, jak ji udr\u017eet zdravou cel\u00e9 m\u011bs\u00edce.<\/p>\n<h2>Co je Petriho miska s agarem?<\/h2>\n<p><a href=\"https:\/\/cs.wikipedia.org\/wiki\/Agar\" target=\"_blank\" rel=\"noopener\">Agar<\/a> je \u017eelatinov\u00e1 l\u00e1tka z mo\u0159sk\u00fdch \u0159as. Kdy\u017e ho sm\u00edch\u00e1te s \u017eivinami, jako je sladov\u00fd extrakt a kvasnice, vytvo\u0159\u00ed pevn\u00fd a v\u00fd\u017eivn\u00fd povrch. Mycelium, vegetativn\u00ed \u010d\u00e1st houby, na n\u011bm dob\u0159e roste. Proto\u017ee na misce vid\u00edte ka\u017ed\u00fd kousek r\u016fstu, snadno odhal\u00edte kontaminaci. D\u00edky tomu m\u016f\u017eete vybrat to nejsiln\u011bj\u0161\u00ed a nej\u010dist\u0161\u00ed mycelium. Misky si m\u016f\u017eete nal\u00e9vat sami, ale pot\u0159ebujete k tomu tlakov\u00fd hrnec a \u010dist\u00e9 pracovi\u0161t\u011b. Na\u0161e hotov\u00e9 <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/petriho-miska-sladovo-kvasnicovy-agar\/\">Petriho misky se sladovo-kvasnicov\u00fdm agarem<\/a> se nal\u00e9vaj\u00ed ve steriln\u00edch podm\u00ednk\u00e1ch, tak\u017ee m\u016f\u017eete za\u010d\u00edt hned.<\/p>\n<h2>Krok 1: za\u010dn\u011bte sporami nebo tk\u00e1n\u00ed<\/h2>\n<p>Postup za\u010d\u00edn\u00e1 sporami nebo mal\u00fdm vzorkem tk\u00e1n\u011b ze zral\u00e9 houby. Ty pak opatrn\u011b p\u0159enesete na steriln\u00ed povrch \u2013 k tomu slou\u017e\u00ed Petriho miska s agarem.<\/p>\n<ul>\n<li><strong>Spory:<\/strong> nejl\u00e9pe kl\u00ed\u010d\u00ed na misce chud\u00e9 na \u017eiviny. Na\u0161e <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/petriho-miska-s-agarem-pro-spory\/\">misky s agarem pro spory<\/a> maj\u00ed lehk\u00fd sladovo-kvasnicov\u00fd recept vyvinut\u00fd pro kl\u00ed\u010den\u00ed.<\/li>\n<li><strong>Tk\u00e1\u0148 (klonov\u00e1n\u00ed):<\/strong> mal\u00fd kousek z vnit\u0159ku zdrav\u00e9 houby v\u00e1m d\u00e1 p\u0159esnou genetickou kopii t\u00e9to houby. V\u00edce se do\u010dtete v na\u0161em n\u00e1vodu na <a href=\"https:\/\/mycosupplies.eu\/cs\/2025\/04\/17\/genetika-hub\/\">zlep\u0161en\u00ed genetiky hub klonov\u00e1n\u00edm<\/a>.<\/li>\n<\/ul>\n<h2>Krok 2: kl\u00ed\u010den\u00ed a prim\u00e1rn\u00ed izolace<\/h2>\n<p>Jakmile spory um\u00edst\u00edte na agar, vykl\u00ed\u010d\u00ed a za\u010dnou tvo\u0159it mycelium. Mycelium se \u010dasem rozroste po misce jako jemn\u00e1 s\u00ed\u0165 b\u00edl\u00fdch nebo barevn\u00fdch vl\u00e1ken. T\u00e9to f\u00e1zi se \u0159\u00edk\u00e1 <strong>prim\u00e1rn\u00ed izolace<\/strong>. B\u011bhem n\u00ed vyb\u00edr\u00e1te \u010d\u00e1sti s nejsiln\u011bj\u0161\u00edm a nejzdrav\u011bj\u0161\u00edm r\u016fstem. Tak m\u016f\u017eete izolovat jedine\u010dn\u00e9 kmeny nebo odr\u016fdy. Po nao\u010dkov\u00e1n\u00ed ka\u017edou misku ut\u011bsn\u011bte <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/parafilm-50-mm-x-5-m\/\">Parafilmem<\/a>: dr\u017e\u00ed ne\u010distoty venku a p\u0159itom umo\u017en\u00ed minim\u00e1ln\u00ed v\u00fdm\u011bnu plyn\u016f, kterou kultura pot\u0159ebuje.<\/p>\n<h2>Krok 3: p\u0159eo\u010dkov\u00e1n\u00ed, aby kultura z\u016fstala \u010dist\u00e1<\/h2>\n<p>Abyste udr\u017eeli \u017eivou kulturu, pravideln\u011b p\u0159en\u00e1\u0161ejte mal\u00fd kousek mycelia na \u010derstvou agarovou misku. Tomuto postupu se \u0159\u00edk\u00e1 <strong>p\u0159eo\u010dkov\u00e1n\u00ed<\/strong> (subkultivace) a udr\u017euje kulturu vit\u00e1ln\u00ed a dlouhov\u011bkou. Mycelium berte v\u017edy z nej\u010dist\u0161\u00edho, p\u0159edn\u00edho okraje r\u016fstu. Tak kontaminaci nech\u00e1te za sebou a udr\u017e\u00edte zdrav\u00fd, \u010dist\u00fd kmen. Pracujte ve stoj\u00edc\u00edm vzduchu, nebo ide\u00e1ln\u011b p\u0159ed <a href=\"\/?product_cat=laminar-flowhoods\">lamin\u00e1rn\u00edm boxem \u010di filtra\u010dn\u00ed jednotkou HEPA<\/a>, a noste <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/nitrilove-rukavice-nepudrovane\/\">nitrilov\u00e9 rukavice<\/a>, abyste sn\u00ed\u017eili riziko kontaminace.<\/p>\n<h2>Krok 4: uchov\u00e1v\u00e1n\u00ed \u017eiv\u00fdch kultur<\/h2>\n<p>Spr\u00e1vn\u00e9 skladov\u00e1n\u00ed je pro \u017eivotnost \u017eiv\u00fdch kultur z\u00e1sadn\u00ed. V\u011bt\u0161ina p\u011bstitel\u016f uchov\u00e1v\u00e1 misky v lednici p\u0159i zhruba 4\u20138 \u00b0C. Chlad zpomaluje mycelium. D\u00edky tomu kultura vydr\u017e\u00ed mnohem d\u00e9le. Ka\u017edou misku nav\u00edc ozna\u010dte kmenem a datem, abyste mohli sledovat svou genetiku.<\/p>\n<h2>Petriho miska s agarem, pak zrno a tekut\u00e1 kultura<\/h2>\n<p>Jakmile m\u00e1te na agaru \u010distou kulturu, m\u016f\u017eete p\u0159ej\u00edt k dal\u0161\u00edmu kroku: p\u0159eneste kl\u00ednek mycelia na <a href=\"https:\/\/mycosupplies.eu\/cs\/kategorie-produktu\/sterilni-zrno-a-substrat\/\">sterilizovan\u00e9 zrno<\/a>, nebo ho namno\u017ete v p\u0159edem sterilizovan\u00e9 <a href=\"https:\/\/mycosupplies.eu\/cs\/produkt\/lahvicka-na-tekutou-kulturu-10-ml\/\">lahvi\u010dce na tekutou kulturu<\/a>. Na agar je\u0161t\u011b nejste p\u0159ipraveni? Na\u0161e hotov\u00e9 <a href=\"https:\/\/mycosupplies.eu\/cs\/kategorie-produktu\/tekuta-kultura\/\">st\u0159\u00edka\u010dky s tekutou kulturou<\/a> obsahuj\u00ed izolovanou genetiku, tak\u017ee m\u016f\u017eete p\u0159ej\u00edt rovnou k o\u010dkov\u00e1n\u00ed.<\/p>\n<h2>Z\u00e1v\u011br<\/h2>\n<p>Petriho miska s agarem je praktick\u00fd zp\u016fsob, jak uchovat obl\u00edben\u00e9 kmeny. Je to tak\u00e9 cenn\u00fd n\u00e1stroj pro pokusy. S ka\u017ed\u00fdm p\u0159enosem se o sv\u00fdch kultur\u00e1ch dozv\u00edte v\u00edc. Zkr\u00e1tka, pr\u00e1ce s agarem je z\u00e1kladn\u00ed dovednost\u00ed ka\u017ed\u00e9ho mykologa.<\/p>\n&#13;\n<p class=\"wp-block-paragraph\">Je\u0161t\u011b nem\u00e1te lamin\u00e1rn\u00ed box? Zjist\u011bte, jak postavit a pou\u017e\u00edvat <a href=\"https:\/\/mycosupplies.eu\/cs\/2026\/10\/23\/still-air-box\/\">still air box<\/a> pro \u010distou pr\u00e1ci s agarem.<\/p>&#13;\n","protected":false},"excerpt":{"rendered":"<p>Jak mykologov\u00e9 p\u011bstuj\u00ed, izoluj\u00ed a uchov\u00e1vaj\u00ed \u017eiv\u00e9 kultury hub: Petriho miska s agarem v\u00e1s dovede od spor \u010di tk\u00e1n\u011b k \u010dist\u00e9 kultu\u0159e, s tipy na p\u0159eo\u010dkov\u00e1n\u00ed a skladov\u00e1n\u00ed.<\/p>\n","protected":false},"author":1,"featured_media":4214,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[816],"tags":[],"class_list":["post-12869","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-pestovani"],"featured_image_src":{"landsacpe":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false],"list":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false],"medium":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4-300x139.jpg",300,139,true],"full":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false]},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.8 (Yoast SEO v28.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Petriho miska s agarem: kultury hub | MycoSupplies<\/title>\n<meta name=\"description\" content=\"Petriho miska s agarem pro kultury hub: od spor \u010di tk\u00e1n\u011b k \u010dist\u00e9 kultu\u0159e, s p\u0159eo\u010dkov\u00e1n\u00edm a skladov\u00e1n\u00edm. 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