{"id":12659,"date":"2018-02-26T07:42:56","date_gmt":"2018-02-26T07:42:56","guid":{"rendered":"https:\/\/mycosupplies.eu\/2018\/02\/26\/placa-de-petri-agar\/"},"modified":"2026-11-12T09:50:53","modified_gmt":"2026-11-12T09:50:53","slug":"placa-de-petri-agar","status":"publish","type":"post","link":"https:\/\/mycosupplies.eu\/es\/2018\/02\/26\/placa-de-petri-agar\/","title":{"rendered":"Cultivos de setas en placa de Petri agar"},"content":{"rendered":"<p>\u00bfTe has preguntado alguna vez c\u00f3mo se cultivan y conservan cultivos vivos de setas en una <strong>placa de Petri agar<\/strong>? El trabajo con agar permite a mic\u00f3logos y aficionados conservar, limpiar y multiplicar sus cepas favoritas. En esta gu\u00eda de la placa de Petri agar te llevamos desde las esporas o el tejido hasta un cultivo sano y limpio. Tambi\u00e9n te mostramos c\u00f3mo mantenerlo sano durante meses.<\/p>\n<h2>\u00bfQu\u00e9 es una placa de Petri agar?<\/h2>\n<p>El <a href=\"https:\/\/es.wikipedia.org\/wiki\/Agar\" target=\"_blank\" rel=\"noopener\">agar<\/a> es una sustancia gelatinosa obtenida de algas. Al mezclarlo con nutrientes como extracto de malta y levadura, forma una superficie firme y nutritiva. El micelio, la parte vegetativa del hongo, crece bien sobre ella. Como ves cada parte del crecimiento en la placa, detectas la contaminaci\u00f3n con facilidad. As\u00ed puedes seleccionar el micelio m\u00e1s fuerte y limpio. Puedes verter tus propias placas, pero necesitas una olla a presi\u00f3n y un espacio limpio. Nuestras <a href=\"https:\/\/mycosupplies.eu\/es\/producto\/placa-petri-agar-malta-levadura\/\">placas de Petri de agar de malta y levadura<\/a> listas para usar se vierten en condiciones est\u00e9riles, para que empieces enseguida.<\/p>\n<h2>Paso 1: empieza con esporas o tejido<\/h2>\n<p>El proceso empieza con esporas o una peque\u00f1a muestra de tejido de una seta madura. Despu\u00e9s la transfieres con cuidado a la superficie est\u00e9ril de una placa de Petri agar.<\/p>\n<ul>\n<li><strong>Esporas:<\/strong> germinan mejor en una placa pobre en nutrientes. Nuestras <a href=\"https:\/\/mycosupplies.eu\/es\/producto\/placa-petri-agar-esporas\/\">placas de agar para esporas<\/a> usan una receta ligera de malta y levadura pensada para la germinaci\u00f3n.<\/li>\n<li><strong>Tejido (clonaci\u00f3n):<\/strong> un trocito del interior de una seta sana te da una copia gen\u00e9tica exacta de esa seta. Lee m\u00e1s en nuestra gu\u00eda para <a href=\"https:\/\/mycosupplies.eu\/es\/2025\/04\/17\/genetica-de-setas\/\">mejorar la gen\u00e9tica de setas mediante clonaci\u00f3n<\/a>.<\/li>\n<\/ul>\n<h2>Paso 2: germinaci\u00f3n y aislamiento primario<\/h2>\n<p>Cuando colocas esporas sobre el agar, germinan y empiezan a formar micelio. Con el tiempo, el micelio se extiende por la placa como una delicada red de filamentos blancos o de color. Esta fase se llama <strong>aislamiento primario<\/strong>. Durante ella, seleccionas las zonas con el crecimiento m\u00e1s vigoroso y sano. As\u00ed puedes aislar cepas o variedades \u00fanicas. Sella cada placa con <a href=\"https:\/\/mycosupplies.eu\/es\/producto\/parafilm-50-mm-x-5-m\/\">Parafilm<\/a> tras inocularla: mantiene fuera los contaminantes y permite el m\u00ednimo intercambio de gases que necesita el cultivo.<\/p>\n<h2>Paso 3: subcultivar para mantener limpio el cultivo<\/h2>\n<p>Para mantener un cultivo vivo, traslada con regularidad un trocito de micelio a una placa de agar nueva. Este proceso se llama <strong>subcultivo<\/strong> y mantiene el cultivo vital y duradero. Toma siempre el micelio del borde de crecimiento m\u00e1s limpio y avanzado. As\u00ed dejas atr\u00e1s la contaminaci\u00f3n y conservas una cepa sana y pura. Trabaja con el aire quieto o, mejor a\u00fan, frente a una <a href=\"\/?product_cat=laminar-flowhoods\">campana de flujo o unidad de filtraci\u00f3n HEPA<\/a>, y usa <a href=\"https:\/\/mycosupplies.eu\/es\/producto\/guantes-de-nitrilo-sin-polvo\/\">guantes de nitrilo<\/a> para reducir el riesgo de contaminaci\u00f3n.<\/p>\n<h2>Paso 4: conservar cultivos vivos<\/h2>\n<p>Una buena conservaci\u00f3n es clave para la vida de los cultivos. La mayor\u00eda de los cultivadores guarda sus placas en la nevera a unos 4\u20138 \u00b0C. La temperatura fr\u00eda ralentiza el micelio. Como resultado, el cultivo dura mucho m\u00e1s. Adem\u00e1s, etiqueta cada placa con la cepa y la fecha para seguir tu gen\u00e9tica.<\/p>\n<h2>Del agar al grano y al cultivo l\u00edquido<\/h2>\n<p>Cuando tengas un cultivo limpio en agar, puedes pasar a la siguiente fase: traslada una cu\u00f1a de micelio a <a href=\"https:\/\/mycosupplies.eu\/es\/categoria-producto\/grano-esterilizado-y-sustrato\/\">grano esterilizado<\/a>, o multipl\u00edcalo en un <a href=\"https:\/\/mycosupplies.eu\/es\/producto\/vial-cultivo-liquido-10-ml\/\">vial de cultivo l\u00edquido<\/a> preesterilizado. \u00bfA\u00fan no te ves con el agar? Nuestras <a href=\"https:\/\/mycosupplies.eu\/es\/categoria-producto\/cultivo-liquido\/\">jeringas de cultivo l\u00edquido<\/a> listas para usar contienen gen\u00e9tica aislada, para que pases directamente a inocular.<\/p>\n<h2>Conclusi\u00f3n<\/h2>\n<p>Una placa de Petri agar es una forma pr\u00e1ctica de conservar tus cepas favoritas. Tambi\u00e9n es una herramienta valiosa para experimentar. Con cada transferencia aprendes m\u00e1s sobre tus cultivos. En resumen, el trabajo con agar es una habilidad esencial para todo mic\u00f3logo.<\/p>\n&#13;\n<p class=\"wp-block-paragraph\">\u00bfA\u00fan no tienes campana de flujo? Aprende a construir y usar una <a href=\"https:\/\/mycosupplies.eu\/es\/2026\/10\/23\/still-air-box\/\">still air box<\/a> para trabajar limpio con agar.<\/p>&#13;\n","protected":false},"excerpt":{"rendered":"<p>C\u00f3mo los mic\u00f3logos cultivan, a\u00edslan y conservan cultivos vivos de setas en placa de Petri agar: de esporas o tejido a un cultivo limpio, con subcultivo y conservaci\u00f3n.<\/p>\n","protected":false},"author":1,"featured_media":4214,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[802],"tags":[],"class_list":["post-12659","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cultivo"],"featured_image_src":{"landsacpe":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false],"list":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false],"medium":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4-300x139.jpg",300,139,true],"full":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false]},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.8 (Yoast SEO v28.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Placa de Petri agar: cultivos de setas | MycoSupplies<\/title>\n<meta name=\"description\" content=\"Placa de Petri agar para cultivos de setas: de esporas o tejido a un cultivo limpio, con subcultivo y conservaci\u00f3n. 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