{"id":12629,"date":"2018-02-26T07:42:56","date_gmt":"2018-02-26T07:42:56","guid":{"rendered":"https:\/\/mycosupplies.eu\/2018\/02\/26\/boite-de-petri-agar\/"},"modified":"2026-11-12T09:51:20","modified_gmt":"2026-11-12T09:51:20","slug":"boite-de-petri-agar","status":"publish","type":"post","link":"https:\/\/mycosupplies.eu\/fr\/2018\/02\/26\/boite-de-petri-agar\/","title":{"rendered":"Cultures de champignons sur bo\u00eete de Petri agar"},"content":{"rendered":"<p>Vous \u00eates-vous d\u00e9j\u00e0 demand\u00e9 comment on cultive et conserve des cultures vivantes de champignons sur une <strong>bo\u00eete de Petri agar<\/strong> ? Le travail sur g\u00e9lose permet aux mycologues et aux amateurs de pr\u00e9server, de nettoyer et de multiplier leurs souches pr\u00e9f\u00e9r\u00e9es. Dans ce guide de la bo\u00eete de Petri agar, nous vous accompagnons des spores ou du tissu jusqu\u2019\u00e0 une culture saine et propre. Nous vous montrons aussi comment la garder en bonne sant\u00e9 pendant des mois.<\/p>\n<h2>Qu\u2019est-ce qu\u2019une bo\u00eete de Petri agar ?<\/h2>\n<p>L\u2019<a href=\"https:\/\/fr.wikipedia.org\/wiki\/Agar-agar\" target=\"_blank\" rel=\"noopener\">agar<\/a> est une substance g\u00e9latineuse extraite d\u2019algues. M\u00e9lang\u00e9 \u00e0 des nutriments comme l\u2019extrait de malt et la levure, il forme une surface ferme et nutritive. Le myc\u00e9lium, la partie v\u00e9g\u00e9tative du champignon, y pousse bien. Comme vous voyez chaque zone de croissance sur la bo\u00eete, vous rep\u00e9rez facilement les contaminations. Vous pouvez ainsi s\u00e9lectionner le myc\u00e9lium le plus vigoureux et le plus propre. Vous pouvez couler vos propres bo\u00eetes, mais il faut alors un autocuiseur et un poste de travail propre. Nos <a href=\"https:\/\/mycosupplies.eu\/fr\/produit\/boite-de-petri-gelose-malt-levure\/\">bo\u00eetes de Petri de g\u00e9lose malt-levure<\/a> pr\u00eates \u00e0 l\u2019emploi sont coul\u00e9es en conditions st\u00e9riles, pour d\u00e9marrer tout de suite.<\/p>\n<h2>\u00c9tape 1 : partir de spores ou de tissu<\/h2>\n<p>Le processus commence avec des spores ou un petit \u00e9chantillon de tissu pr\u00e9lev\u00e9 sur un champignon m\u00fbr. Vous les transf\u00e9rez ensuite avec pr\u00e9caution sur la surface st\u00e9rile d\u2019une bo\u00eete de Petri agar.<\/p>\n<ul>\n<li><strong>Spores :<\/strong> elles germent mieux sur une bo\u00eete pauvre en nutriments. Nos <a href=\"https:\/\/mycosupplies.eu\/fr\/produit\/boite-de-petri-gelose-spores\/\">bo\u00eetes de g\u00e9lose pour spores<\/a> utilisent une recette l\u00e9g\u00e8re malt-levure con\u00e7ue pour la germination.<\/li>\n<li><strong>Tissu (clonage) :<\/strong> un petit morceau pr\u00e9lev\u00e9 \u00e0 l\u2019int\u00e9rieur d\u2019un champignon sain vous donne une copie g\u00e9n\u00e9tique exacte de ce champignon. Pour en savoir plus, lisez notre guide pour <a href=\"https:\/\/mycosupplies.eu\/fr\/2025\/04\/17\/genetique-des-champignons\/\">am\u00e9liorer la g\u00e9n\u00e9tique des champignons par clonage<\/a>.<\/li>\n<\/ul>\n<h2>\u00c9tape 2 : germination et isolement primaire<\/h2>\n<p>Une fois pos\u00e9es sur la g\u00e9lose, les spores germent et commencent \u00e0 former du myc\u00e9lium. Avec le temps, le myc\u00e9lium s\u2019\u00e9tend sur la bo\u00eete en un fin r\u00e9seau de filaments blancs ou color\u00e9s. Cette \u00e9tape s\u2019appelle l\u2019<strong>isolement primaire<\/strong>. Vous s\u00e9lectionnez alors les zones \u00e0 la croissance la plus vigoureuse et la plus saine. Vous pouvez ainsi isoler des souches ou vari\u00e9t\u00e9s uniques. Fermez chaque bo\u00eete avec du <a href=\"https:\/\/mycosupplies.eu\/fr\/produit\/parafilm-50-mm-x-5-m\/\">Parafilm<\/a> apr\u00e8s l\u2019inoculation : il emp\u00eache les contaminants d\u2019entrer tout en permettant les \u00e9changes gazeux minimaux dont la culture a besoin.<\/p>\n<h2>\u00c9tape 3 : repiquer pour garder une culture propre<\/h2>\n<p>Pour entretenir une culture vivante, transf\u00e9rez r\u00e9guli\u00e8rement un petit morceau de myc\u00e9lium sur une bo\u00eete de g\u00e9lose neuve. Ce proc\u00e9d\u00e9 s\u2019appelle le <strong>repiquage<\/strong> et garde la culture vigoureuse et durable. Pr\u00e9levez toujours le myc\u00e9lium sur le front de croissance le plus propre. Vous laissez ainsi la contamination derri\u00e8re vous et conservez une souche saine et pure. Travaillez en air calme ou, id\u00e9alement, devant une <a href=\"\/?product_cat=laminar-flowhoods\">hotte \u00e0 flux laminaire ou une unit\u00e9 de filtration HEPA<\/a>, et portez des <a href=\"https:\/\/mycosupplies.eu\/fr\/produit\/gants-nitrile-sans-poudre\/\">gants en nitrile<\/a> pour r\u00e9duire le risque de contamination.<\/p>\n<h2>\u00c9tape 4 : conserver des cultures vivantes<\/h2>\n<p>Une bonne conservation est cruciale pour la long\u00e9vit\u00e9 des cultures vivantes. La plupart des cultivateurs gardent leurs bo\u00eetes au r\u00e9frig\u00e9rateur \u00e0 environ 4\u20138 \u00b0C. La fra\u00eecheur ralentit le myc\u00e9lium. La culture dure donc beaucoup plus longtemps. \u00c9tiquetez aussi chaque bo\u00eete avec la souche et la date pour suivre votre g\u00e9n\u00e9tique.<\/p>\n<h2>De la g\u00e9lose aux grains et \u00e0 la culture liquide<\/h2>\n<p>Une fois que vous avez une culture propre sur g\u00e9lose, vous pouvez passer \u00e0 l\u2019\u00e9tape suivante : transf\u00e9rez un morceau de myc\u00e9lium sur des <a href=\"https:\/\/mycosupplies.eu\/fr\/categorie-produit\/grain-sterilise-et-substrat\/\">grains st\u00e9rilis\u00e9s<\/a>, ou multipliez-le dans un <a href=\"https:\/\/mycosupplies.eu\/fr\/produit\/flacon-culture-liquide-10-ml\/\">flacon de culture liquide<\/a> pr\u00e9st\u00e9rilis\u00e9. Pas encore pr\u00eat pour la g\u00e9lose ? Nos <a href=\"https:\/\/mycosupplies.eu\/fr\/categorie-produit\/culture-liquide\/\">seringues de culture liquide<\/a> pr\u00eates \u00e0 l\u2019emploi contiennent une g\u00e9n\u00e9tique isol\u00e9e, pour passer directement \u00e0 l\u2019inoculation.<\/p>\n<h2>Conclusion<\/h2>\n<p>Une bo\u00eete de Petri agar est un moyen pratique de pr\u00e9server vos souches pr\u00e9f\u00e9r\u00e9es. C\u2019est aussi un outil pr\u00e9cieux pour exp\u00e9rimenter. \u00c0 chaque transfert, vous en apprenez davantage sur vos cultures. Bref, le travail sur g\u00e9lose est une comp\u00e9tence essentielle pour tout mycologue.<\/p>\n&#13;\n<p class=\"wp-block-paragraph\">Pas encore de hotte \u00e0 flux laminaire ? D\u00e9couvrez comment fabriquer et utiliser une <a href=\"https:\/\/mycosupplies.eu\/fr\/2026\/10\/23\/still-air-box\/\">still air box<\/a> pour un travail propre sur g\u00e9lose.<\/p>&#13;\n","protected":false},"excerpt":{"rendered":"<p>Comment les mycologues cultivent, isolent et conservent des cultures vivantes sur bo\u00eete de Petri agar : des spores ou du tissu \u00e0 une culture propre, avec repiquage et conservation.<\/p>\n","protected":false},"author":1,"featured_media":4214,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[800],"tags":[],"class_list":["post-12629","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-culture"],"featured_image_src":{"landsacpe":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false],"list":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false],"medium":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4-300x139.jpg",300,139,true],"full":["https:\/\/mycosupplies.eu\/wp-content\/uploads\/2018\/02\/rhizoplate-4.jpg",400,185,false]},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.8 (Yoast SEO v28.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Bo\u00eete de Petri agar : cultures de champignons | MycoSupplies<\/title>\n<meta name=\"description\" content=\"Bo\u00eete de Petri agar pour cultures de champignons : des spores ou du tissu \u00e0 une culture propre, avec repiquage et conservation. 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